Immunomedicine Group: Tools >> BEPIBLAST: B Cell Epitope Prediction using a BLAST-based module

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>> BEPIBLAST: B cell EPItope prediction using a BLAST-based module

Input	Paste Sequence in Fasta format (Replace Example)
	>sp\|P03437\|HEMA_I68A0 Hemagglutinin OS=Influenza A virus (strain A/Aichi/2/1968 H3N2) OX=387139 GN=HA PE=1 SV=1 MKTIIALSYIFCLALGQDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGIHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEEMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKDWILWISFAISCFLLCVVLLGFIMWACQRGNIRCNICI
	Or upload fasta file:

Search Epitopes

Epitope Feature

Peptide length:

Output options

Only neutralizing
Map in sequence

Min. Identity ≥

%

Gaps:

Allowed

Citation:

Ras-Carmona A, Lehmann AA, Lehmann PV & Reche PA (2022). Prediction of B cell epitopes in proteins using a novel sequence similarity-based method. Sci Rep, 12(1), 13739. doi: 10.1038/s41598-022-18021-1

Hits since March/2022

Last updated:


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HELP: Epitope Features

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Here the user can select the parameters that the alignment must meet to consider a sequence fragment (target sequence) as a B-cell epitope. Given the default parameters, here we present an example of an alignment that would result in the prediction of a linear B-cell epitope. Likewise, we show some alignments that would be descated and not considerated as positive hits:

HELP: Output options

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If the option "Only neutralizing" is selected, BepiBlast, will only return B cell epitopes resulting from hits to neutralizing epitopes. If the option "Map in sequence" is selected, BepiBlast colors the sequence residues into an RGB scale, which enable mapping B cell epitopes in the query seqeunce.

DEVELOPERS

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	Alvaro Ras Carmona, PhD Student

	Dr. Pedro A Reche, Group Leader

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