Detailed Help

Sequence Information

Input query for DACHIS can either be a protein sequence in plane or standard formats such as Fasta,EMBL,GCG, etc. Input query can be pasted or uplo aded from a local file. If a local file is uploaded, it will be processed by default. Files uploaded onto the server must contain only ascii characte rs. In other words, make sure you save your files as TEXT before you make any attempt of uploading them onto this server. Otherwise, your computer mi ght, literally, exploit in front of your nose. Please, note that the MIF will not liable for any personal injury derived from such m issuse of the server..

Prediction Approaches

The method allow the prediction on the basis of two different appraoches.

1. Composition of amino acids:

   A SVM was developed on the basis of composition amino acids of protein. The SVM was provided with a 20 dimensional vector. The amino acid composition is fraction of each amino acid in a protein.The performence of the method is evalvuated using fiv e fold cross-validation.

2. Dipeptide composition:

   A SVM was developed on the basis of composition of dipeptides of protein sequence. This will give a fixed pattern length of 400. This representation encompasses the information about amino acid composition along local order of amino acid.

Prediction Results

The prediction results are presented in very user friendly format. The results are consist of mainly two parts.

• Summary of query sequence:

  This part provides the information about the submitted sequence like the sequence, length of sequence and date of scanning. This part also provides the information about the choosen prediction approach.

• Results:

  This part provides information about the final predicted type of amine receptor. It also provides information about reliability of prediction (in form of reliability index) and expected accuracy.